小鼠腫瘤接種模型，時(shí)間一般持續(xù)2-3周。如何使用氯膦酸二鈉脂質(zhì)體清除小鼠腫瘤模型巨噬細(xì)胞？對(duì)于這種時(shí)間長的疾病模型，小鼠的狀態(tài)，小鼠的存活，比清除效果更為重要。兩者的平衡或者偏向，需要?jiǎng)討B(tài)調(diào)整。

如下這篇Nature Communications，使用荷蘭Liposoma品牌巨噬細(xì)胞清除劑氯膦酸二鈉脂質(zhì)體：Clodronate Liposomes&Control Liposomes。小鼠疾病模型：LLC肺癌模型。LLC細(xì)胞全稱為Lewis肺癌細(xì)胞（Lewis Lung Carcinoma），是一種來源于小鼠的肺癌細(xì)胞系，常用于癌癥研究的動(dòng)物模型中。它并非人類肺癌的臨床分型，而是實(shí)驗(yàn)室中用于模擬肺癌生長、轉(zhuǎn)移及藥物測試的模型工具。腹腔注射200ul，在腫瘤細(xì)胞接種后第7天，11天，15天腹腔注射荷蘭Liposoma品牌巨噬細(xì)胞清除劑氯膦酸二鈉脂質(zhì)體：Clodronate Liposomes&Control Liposomes（CP-005-005）。

氯膦酸二鈉脂質(zhì)體清除小鼠腫瘤模型巨噬細(xì)胞效果檢測：

a Schematic of clodronate and control liposome treatment of LLC tumor bearing mice. b, c LLC tumor volumes over time (b) and endpoint tumor volumes (c) for WT (n?=?6 mice treated with control liposomes (con) and 8 mice treated with clodronate liposomes (clod) and for Mlck210?/? [n?=?7 mice treated with control liposomes (con) and clodronate liposomes (clod)]. One WT animal treated with clodronate liposomes died on day 14 post-tumor inoculation and was not included in the final tumor weight graph. d–g Quantification (d, f) and FACs plots of Ly6C- (e) liver and (g) tumor macrophages from WT and Mlck210?/? mice after treatment with clodronate and control liposomes (n?=?4 mice).

論文信息：

論文題目：PI3Kγ stimulates a high molecular weight form of myosin light chain kinase to promote myeloid cell adhesion and tumor inflammation

期刊名稱：Nature Communications

時(shí)間期卷：13, Article number: 1768 (2022)

在線時(shí)間：2022年4月1日

DOI：doi.org/10.1038/s41467-022-29471-6

產(chǎn)品信息：

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱：Clodronate Liposomes&Control Liposomes

辦事處：Target Technology(靶點(diǎn)科技)

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體的材料和方法：

Cell depletion

In other experiments, wildtype female C57BL6 or Mlck210?/? mice in the C57BL6 background bearing LLC tumors (n?=?6–8) were treated with daily i.p. injections of 1?mg clodronate or control liposomes (Liposoma Research Liposomes # CP-005-005) in 200?µl on day 7,11 and 15 after tumor inoculation. Tumors dimensions were recorded at regular intervals, typically every 1–2 days. Tumors were excised at 18 days after implantation and tumors, spleens and livers were excised for further analysis by flow cytometry. Alternatively, WT and Mlck210?/? C57Bl6 male mice bearing HPV+ MEER tumors (n?=?8–11) were treated with i.p. injections of 100?µg anti-CD8 antibodies (BioXcell In Vivo Plus Clone YTS 169.4, #BE0117) or saline on days 27, 29, 32, 41, and 44 after tumor inoculation. Tumor volumes were measured every 2–3 days. Tumors and spleens were harvested on day 48 after tumor inoculation for flow cytometry analysis.